I just had my last midterm Molecular Biology quiz. Let me share with you some Molecular concept and definitions:
An element with the consensus sequence TATAAAA that begins about 30 bp upstream of the start of transcription most eukaryotic promoters recognized by RNA polymerase II.
RNA Polymerase II Holenezyme in prokaryotes
The collection of polypeptides that make up the whole enzyme. Usually includes, Beta, 'Beta, 2xAlpha, Sigma subunits.
A specific pattern of peptide spots formed when a protein is cut into pieces with an enzyme, and then the peptides are separated by chromatography.
The Concept of DNA Replication
1- DNA Replication is Semi-conservative. That is, replications give two daughters duplex DNAs each contain an old strand (Parent) and new strand (Daughter)
2- DNA Replication is Semi-continuous. That is, DNA polymerase makes one strand continuous (leading strand):
But the other strand (lagging strand) would have to be discontinuous:
'3------>'5 (AKA. Okazaki fragments)
3- DNA Replication needs Primer. That is, a Primer is needed to start DNA synthesis by making short pieces of RNA on DNA strands.
4- DNA Replication is Bidirectional. That is, In the Origin of Replication, two forks arise and move in opposite directions around the circle until they meet on the other side.
Okay, I have covered only 0.3% of the test material.
Posted by Angelo
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