Molecular Stuff

I just had my last midterm Molecular Biology quiz. Let me share with you some Molecular concept and definitions:

An element with the consensus sequence TATAAAA that begins about 30 bp upstream of the start of transcription most eukaryotic promoters recognized by RNA polymerase II.

RNA Polymerase II Holenezyme in prokaryotes
The collection of polypeptides that make up the whole enzyme. Usually includes, Beta, 'Beta, 2xAlpha, Sigma subunits.

Footprint Essay
A specific pattern of peptide spots formed when a protein is cut into pieces with an enzyme, and then the peptides are separated by chromatography.

The Concept of DNA Replication
1- DNA Replication is Semi-conservative. That is, replications give two daughters duplex DNAs each contain an old strand (Parent) and new strand (Daughter)

2- DNA Replication is Semi-continuous. That is, DNA polymerase makes one strand continuous (leading strand):
But the other strand (lagging strand) would have to be discontinuous:
'3------>'5 (AKA. Okazaki fragments)

3- DNA Replication needs Primer. That is, a Primer is needed to start DNA synthesis by making short pieces of RNA on DNA strands.

4- DNA Replication is Bidirectional. That is, In the Origin of Replication, two forks arise and move in opposite directions around the circle until they meet on the other side.

Okay, I have covered only 0.3% of the test material.

8 Responses to "Molecular Stuff"

PhotoFlo (visit their site)

That was my favorite clas of the whole moleculart and cellular biology major. I always get a kick out of the TATA box..

Fallen Angel (visit their site)

You really think so? For me, I loved the genetic class more..

LaiaLy_q8 (visit their site)

ew :p

business is the way to go baby :p

PhotoFlo (visit their site)

Ew I hated genetics, we had the worst teachers on earth!

Fallen Angel (visit their site)

@ laialy_q8: LoL this is so typical. Like every kuwaiti on earth is majoring in either business or engineering.

@ photoflow: I blame the teacher not the course cuz genetics is sooo much fun. I particularly analyzed the pedigree of our family true and was able to find out that I am carrier of "fe8er dam".

lawyer (visit their site)

good luck enshalla
as 4 me ana ma3ref about ur major wayed

alah ya3tech al3afya

Fallen Angel (visit their site)

@ lawyer: Thank You so much ^_^

I'm also double-majoring in Psychology also.
O allah ye3afech ;)

Ms.Baker (visit their site)

Dear Fallen Angel,

Molecular biology and genetics are amazing... :) DNA replication is such a beautiful little coordinated symphonic dance.